STAR: Problem with generating BAM file
Hi Alex,
I got a problem while generating BAM files using STAR 2.7.9a. The error message was following:
EXITING because of FATAL ERROR: number of bytes expected from the BAM bin does not agree with the actual size on disk: Expected bin size=9792429096 ; size on disk=0 ; bin number=46
The commands I used are following:
for ID in cat list.txt do mkdir star_${ID} star --runThreadN 4 \ --limitBAMsortRAM 31000000000 \ --genomeDir genome \ --outFileNamePrefix ${ID}_ \ --quantMode TranscriptomeSAM \ --outSAMtype BAM SortedByCoordinate \ --readFilesIn ${ID}_trim_pair_1.fastq.gz ${ID}_trim_pair_2.fastq.gz \ --readFilesCommand gunzip -c done
There was actually enough space to run them (>400 GB). I also did ulimit -n 10000
. I successfully completed the mapping with test fastq.gz files which were small files before.
Could you please help me correct the error? Thank you so much!
About this issue
- Original URL
- State: open
- Created 3 years ago
- Comments: 17 (7 by maintainers)
Hi Alex, I think the fastq was not sorted before running STAR. I only processed it using Trimmomatic for adaptor and low quality trimming. I tried
ulimit -n 10000
but the same issue occurred.I will do it with
samtools
as you suggested at this time.Thanks a lot.